Hemopoietic support capacity of adult mouse liver. Studies in 89 Sr marrow-ablated mice

Hemopoietic support capacity of adult mouse liver. Studies in 89 Sr marrow-ablated mice.

The capacity of normal livers in adult mice to support proliferation of pluripotent hemopoietic stem cells (CFU-S) was studied. We assayed CFU-S of the blood and livers of mice with intact marrows and of mice whose marrows had been ablated with “Sr (4 zCi/g) either before or after removal of their spleens, the major hemopoietic organ in marrow-ablated mice. Splenectomy alone resulted in an incr...

Comparison of Neonatal and Adult Mice-derived Sertoli Cells in Support of Expansion of Mouse Spermatogonial Stem Cells In vitro

Separation of hemopoietic cells from adult mouse marrow by use of monoclonal antibodies.

Primitive hemopoietic progenitor cells from adult mouse marrow have been substantially enriched by virtue of a negative selection procedure with monoclonal antibodies. It has been possible to segregate erythroid progenitor cells at distinct stages of differentiation on the basis of their cell surface antigens. This has been achieved with two monoclonal antibodies reactive with the mature elemen...

Therapeutic Potential of Mouse Bone Marrow Mesenchymal Stem Cells in Carbon Tetrachloride (Ccl4)-Induced Liver Fibrosis

Purpose: To study the effect of allogenic bone marrow mesenchymal stem cells (BMMSCs) transplantation on carbon tetrachloride-induced liver fibrosis in mice. Materials and Methods: Fifty five female NMRI mice were divided in 5 groups, and to induce liver fibrosis CCL4 intraperitonealy was injected 1ml/Kg twice a week for 8 weeks 106 allogenic BMMSCs were infused in cell therapy group via tail v...

Autophagy-Modulated Human Bone Marrow-Derived Mesenchymal Stem Cells Accelerate Liver Restoration in Mouse Models of Acute Liver Failure

Background: Mesenchymal stem cells (MSCs) have been recently received increasing attention for cell-based therapy, especially in regenerative medicine. However, the low survival rate of these cells restricts their therapeutic applications. It is hypothesized that autophagy might play an important role in cellular homeostasis and survival. This study aims to investigate the regenerative potentia...